by C M Brown and D K Layman
Abstract:
The relationships of tissue and plasma lipoprotein lipase (LPL) activities to tissue uptake and plasma clearance of 14C-labeled chylomicron-triglyceride (14C-CM-TG) were studied in female rats fed isoenergetic and isonitrogenous control (12% kJ from fat) or high fat diets (72% kJ from fat) for 8 wk. Animals fed the high-fat diet had higher levels of fasting plasma triglycerides and lower LPL activities in heart, renal adipose tissue and post-heparin plasma. Changes in LPL activities of skeletal muscles varied among muscles with higher values in the soleus and plantaris (32-61%) and no differences in the gastrocnemius. The lower LPL activity in renal adipose tissue was associated with lower uptake of fatty acids from 14C-CM-TG by adipose. Fatty-acid uptake from labeled TG was not associated with tissue LPL activity in other tissues. Clearance of 14C-CM-TG from plasma and the half-lives of 14C-CM-TG were similar in both dietary groups. These data indicate that tissue and plasma LPL activities are not a direct index of uptake of fatty acids by tissues or clearance of chylomicron triglycerides.
Reference:
Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet (C M Brown and D K Layman), In J Nutr, volume 118, 1988.
Bibtex Entry:
@article{Brown:1988aa,
abstract = {The relationships of tissue and plasma lipoprotein lipase (LPL) activities to tissue uptake and plasma clearance of 14C-labeled chylomicron-triglyceride (14C-CM-TG) were studied in female rats fed isoenergetic and isonitrogenous control (12% kJ from fat) or high fat diets (72% kJ from fat) for 8 wk. Animals fed the high-fat diet had higher levels of fasting plasma triglycerides and lower LPL activities in heart, renal adipose tissue and post-heparin plasma. Changes in LPL activities of skeletal muscles varied among muscles with higher values in the soleus and plantaris (32-61%) and no differences in the gastrocnemius. The lower LPL activity in renal adipose tissue was associated with lower uptake of fatty acids from 14C-CM-TG by adipose. Fatty-acid uptake from labeled TG was not associated with tissue LPL activity in other tissues. Clearance of 14C-CM-TG from plasma and the half-lives of 14C-CM-TG were similar in both dietary groups. These data indicate that tissue and plasma LPL activities are not a direct index of uptake of fatty acids by tissues or clearance of chylomicron triglycerides.},
author = {Brown, C M and Layman, D K},
date-added = {2023-07-23 20:37:13 +0100},
date-modified = {2023-07-23 20:37:13 +0100},
doi = {10.1093/jn/118.11.1294},
journal = {J Nutr},
journal-full = {The Journal of nutrition},
keywords = {Protein Synthesis},
mesh = {Animals; Carbon Radioisotopes; Chylomicrons; Dietary Fats; Fatty Acids; Female; Lipoprotein Lipase; Metabolic Clearance Rate; Rats; Rats, Inbred Strains; Tissue Distribution; Triglycerides},
month = {Nov},
number = {11},
pages = {1294-8},
pmid = {3193247},
pst = {ppublish},
title = {Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet},
volume = {118},
year = {1988},
bdsk-url-1 = {https://doi.org/10.1093/jn/118.11.1294}}