by Y H Lee, D K Layman, R R Bell and H W Norton
Abstract:
Glutathione peroxidase (GSH-Px) and catalase activities were evaluated during intake of excess dietary iron. Male Sprague-Dawley rats were randomized into seven dietary treatments. The treatments included three levels of dietary iron (35, 305, and 1255 ppm) plus deficiencies of Se or Se and vitamin E at the two high iron levels. Lipid peroxidation in liver and GSH-Px and catalase activities in erythrocytes and liver were measured. Lipid peroxidation was elevated in all high iron groups compared to controls. Total GSH-Px in erythrocytes and liver remained constant or decreased in animals receiving high iron, but non Se GSH-Px increased significantly in liver from rats fed high iron (305 ppm: 155% and 1255 ppm: 131%) and increased additionally in Se and vitamin E deficient groups. No differences in RBC catalase activity were observed. Liver catalase activity increased at least 72% during deficiencies of Se and vitamin E. In summary, GSH-Px did not respond to increased oxidative stress associated with elevated dietary iron except for the non Se GSH-Px which accounts for a relatively small amount of total activity in liver. Catalase increased in liver only when GSH-Px and vitamin E are limiting.
Reference:
Response of glutathione peroxidase and catalase to excess dietary iron in rats (Y H Lee, D K Layman, R R Bell and H W Norton), In J Nutr, volume 111, 1981.
Bibtex Entry:
@article{Lee:1981aa,
abstract = {Glutathione peroxidase (GSH-Px) and catalase activities were evaluated during intake of excess dietary iron. Male Sprague-Dawley rats were randomized into seven dietary treatments. The treatments included three levels of dietary iron (35, 305, and 1255 ppm) plus deficiencies of Se or Se and vitamin E at the two high iron levels. Lipid peroxidation in liver and GSH-Px and catalase activities in erythrocytes and liver were measured. Lipid peroxidation was elevated in all high iron groups compared to controls. Total GSH-Px in erythrocytes and liver remained constant or decreased in animals receiving high iron, but non Se GSH-Px increased significantly in liver from rats fed high iron (305 ppm: 155% and 1255 ppm: 131%) and increased additionally in Se and vitamin E deficient groups. No differences in RBC catalase activity were observed. Liver catalase activity increased at least 72% during deficiencies of Se and vitamin E. In summary, GSH-Px did not respond to increased oxidative stress associated with elevated dietary iron except for the non Se GSH-Px which accounts for a relatively small amount of total activity in liver. Catalase increased in liver only when GSH-Px and vitamin E are limiting.},
author = {Lee, Y H and Layman, D K and Bell, R R and Norton, H W},
date-added = {2023-07-23 20:37:13 +0100},
date-modified = {2023-07-23 20:37:13 +0100},
doi = {10.1093/jn/111.12.2195},
journal = {J Nutr},
journal-full = {The Journal of nutrition},
keywords = {Protein Synthesis},
mesh = {Animals; Catalase; Diet; Erythrocytes; Glutathione Peroxidase; Iron; Liver; Male; Malondialdehyde; Peroxidases; Rats; Rats, Inbred Strains; Selenium; Vitamin E; Vitamin E Deficiency},
month = {Dec},
number = {12},
pages = {2195-202},
pmid = {7310544},
pst = {ppublish},
title = {Response of glutathione peroxidase and catalase to excess dietary iron in rats},
volume = {111},
year = {1981},
bdsk-url-1 = {https://doi.org/10.1093/jn/111.12.2195}}