by Madonna M Mamerow, Joni A Mettler, Kirk L English, Shanon L Casperson, Emily Arentson-Lantz, Melinda Sheffield-Moore, Donald K Layman and Douglas Paddon-Jones
Abstract:
The RDA for protein describes the quantity that should be consumed daily to meet population needs and to prevent deficiency. Protein consumption in many countries exceeds the RDA; however, intake is often skewed toward the evening meal, whereas breakfast is typically carbohydrate rich and low in protein. We examined the effects of protein distribution on 24-h skeletal muscle protein synthesis in healthy adult men and women (n = 8; age: 36.9 $\pm$ 3.1 y; BMI: 25.7 $\pm$ 0.8 kg/m2). By using a 7-d crossover feeding design with a 30-d washout period, we measured changes in muscle protein synthesis in response to isoenergetic and isonitrogenous diets with protein at breakfast, lunch, and dinner distributed evenly (EVEN; 31.5 $\pm$ 1.3, 29.9 $\pm$ 1.6, and 32.7 $\pm$ 1.6 g protein, respectively) or skewed (SKEW; 10.7 $\pm$ 0.8, 16.0 $\pm$ 0.5, and 63.4 $\pm$ 3.7 g protein, respectively). Over 24-h periods on days 1 and 7, venous blood samples and vastus lateralis muscle biopsy samples were obtained during primed (2.0 μmol/kg) constant infusion [0.06 μmol/(kg⋅min)] of l-[ring-(13)C6]phenylalanine. The 24-h mixed muscle protein fractional synthesis rate was 25% higher in the EVEN (0.075 $\pm$ 0.006%/h) vs. the SKEW (0.056 $\pm$ 0.006%/h) protein distribution groups (P = 0.003). This pattern was maintained after 7 d of habituation to each diet (EVEN vs. SKEW: 0.077 $\pm$ 0.006 vs. 0.056 $\pm$ 0.006%/h; P = 0.001). The consumption of a moderate amount of protein at each meal stimulated 24-h muscle protein synthesis more effectively than skewing protein intake toward the evening meal.
Reference:
Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults (Madonna M Mamerow, Joni A Mettler, Kirk L English, Shanon L Casperson, Emily Arentson-Lantz, Melinda Sheffield-Moore, Donald K Layman and Douglas Paddon-Jones), In J Nutr, volume 144, 2014.
Bibtex Entry:
@article{Mamerow:2014aa,
abstract = {The RDA for protein describes the quantity that should be consumed daily to meet population needs and to prevent deficiency. Protein consumption in many countries exceeds the RDA; however, intake is often skewed toward the evening meal, whereas breakfast is typically carbohydrate rich and low in protein. We examined the effects of protein distribution on 24-h skeletal muscle protein synthesis in healthy adult men and women (n = 8; age: 36.9 $\pm$ 3.1 y; BMI: 25.7 $\pm$ 0.8 kg/m2). By using a 7-d crossover feeding design with a 30-d washout period, we measured changes in muscle protein synthesis in response to isoenergetic and isonitrogenous diets with protein at breakfast, lunch, and dinner distributed evenly (EVEN; 31.5 $\pm$ 1.3, 29.9 $\pm$ 1.6, and 32.7 $\pm$ 1.6 g protein, respectively) or skewed (SKEW; 10.7 $\pm$ 0.8, 16.0 $\pm$ 0.5, and 63.4 $\pm$ 3.7 g protein, respectively). Over 24-h periods on days 1 and 7, venous blood samples and vastus lateralis muscle biopsy samples were obtained during primed (2.0 μmol/kg) constant infusion [0.06 μmol/(kg⋅min)] of l-[ring-(13)C6]phenylalanine. The 24-h mixed muscle protein fractional synthesis rate was 25% higher in the EVEN (0.075 $\pm$ 0.006%/h) vs. the SKEW (0.056 $\pm$ 0.006%/h) protein distribution groups (P = 0.003). This pattern was maintained after 7 d of habituation to each diet (EVEN vs. SKEW: 0.077 $\pm$ 0.006 vs. 0.056 $\pm$ 0.006%/h; P = 0.001). The consumption of a moderate amount of protein at each meal stimulated 24-h muscle protein synthesis more effectively than skewing protein intake toward the evening meal.},
author = {Mamerow, Madonna M and Mettler, Joni A and English, Kirk L and Casperson, Shanon L and Arentson-Lantz, Emily and Sheffield-Moore, Melinda and Layman, Donald K and Paddon-Jones, Douglas},
date-added = {2023-07-23 20:34:34 +0100},
date-modified = {2023-07-23 20:34:34 +0100},
doi = {10.3945/jn.113.185280},
journal = {J Nutr},
journal-full = {The Journal of nutrition},
keywords = {Protein Synthesis},
mesh = {Adult; Body Mass Index; Cross-Over Studies; Diet; Dietary Carbohydrates; Dietary Proteins; Energy Intake; Female; Healthy Volunteers; Humans; Male; Meals; Middle Aged; Muscle Proteins; Phenylalanine; Quadriceps Muscle; Tissue Distribution},
month = {Jun},
number = {6},
pages = {876-80},
pmc = {PMC4018950},
pmid = {24477298},
pst = {ppublish},
title = {Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults},
volume = {144},
year = {2014},
bdsk-url-1 = {https://doi.org/10.3945/jn.113.185280}}