by L M Rosch, A R Sherman and D K Layman
Abstract:
To determine if decreased protein synthesis is a factor in reduced immunocompetence of iron deficiency, RNA, DNA and in vitro protein synthesis were measured. Rats were fed diets containing 6 (severe anemia), 11 (moderate anemia) or 250 (iron sufficient) mg iron/kg diet throughout gestation and lactation. On d 2 of lactation, litters were adjusted to contain six pups. On d 12 of lactation, two pups from each litter were immunized with sheep red blood cells (SRBC) and on d 17, tissues were removed for the determination of protein synthesis and evaluation of RNA and DNA contents. In the moderately iron-deficient pups, protein synthesis was lower (30%) in spleen than that in iron-sufficient pups. Protein synthesis in liver and thymus was not changed by moderate iron deficiency. In spleen, liver and thymus, protein synthesis in severely iron-deficient pups was less than half that of iron-sufficient pups. Protein synthesis in the spleen of the moderately iron-deficient group was higher after immunization with SRBC than in iron-sufficient controls, whereas the severely iron-deficient pups failed to respond. Impaired protein synthesis may be the mechanism responsible for compromised ability to produce antibody in iron deficiency.
Reference:
Iron deficiency impairs protein synthesis in immune tissues of rat pups (L M Rosch, A R Sherman and D K Layman), In J Nutr, volume 117, 1987.
Bibtex Entry:
@article{Rosch:1987aa,
abstract = {To determine if decreased protein synthesis is a factor in reduced immunocompetence of iron deficiency, RNA, DNA and in vitro protein synthesis were measured. Rats were fed diets containing 6 (severe anemia), 11 (moderate anemia) or 250 (iron sufficient) mg iron/kg diet throughout gestation and lactation. On d 2 of lactation, litters were adjusted to contain six pups. On d 12 of lactation, two pups from each litter were immunized with sheep red blood cells (SRBC) and on d 17, tissues were removed for the determination of protein synthesis and evaluation of RNA and DNA contents. In the moderately iron-deficient pups, protein synthesis was lower (30%) in spleen than that in iron-sufficient pups. Protein synthesis in liver and thymus was not changed by moderate iron deficiency. In spleen, liver and thymus, protein synthesis in severely iron-deficient pups was less than half that of iron-sufficient pups. Protein synthesis in the spleen of the moderately iron-deficient group was higher after immunization with SRBC than in iron-sufficient controls, whereas the severely iron-deficient pups failed to respond. Impaired protein synthesis may be the mechanism responsible for compromised ability to produce antibody in iron deficiency.},
author = {Rosch, L M and Sherman, A R and Layman, D K},
date-added = {2023-07-23 20:37:13 +0100},
date-modified = {2023-07-23 20:37:13 +0100},
doi = {10.1093/jn/117.8.1475},
journal = {J Nutr},
journal-full = {The Journal of nutrition},
keywords = {Protein Synthesis},
mesh = {Animals; Animals, Newborn; Body Weight; DNA; Erythrocytes; Female; Immune System; Immunization; Iron Deficiencies; Lactation; Liver; Male; Pregnancy; Pregnancy Complications; Protein Biosynthesis; RNA; Rats; Rats, Inbred Strains; Sheep; Spleen; Thymus Gland},
month = {Aug},
number = {8},
pages = {1475-81},
pmid = {2442329},
pst = {ppublish},
title = {Iron deficiency impairs protein synthesis in immune tissues of rat pups},
volume = {117},
year = {1987},
bdsk-url-1 = {https://doi.org/10.1093/jn/117.8.1475}}